12/18/2023 0 Comments Abbott diagnostics dengueIn addition, multiple indirect immunofluorescence assays (IFA, Flavivirus Mosaic 2 or Arboviral Fever Mosaic-2, IgM and IgG (Euroimmun, Lübeck, Germany) were performed for specific IgM and IgG titers measurement and differential diagnosis. Two molecular assays to detect DENV RNA were used: a real time RT-PCR (DENV-1-4 Real-Time RT-PCR Multiplex Assay, developed in 2019 by Centers for Disease Control and Prevention (CDC, Atlanta, GA, USA), and a modified Pan-flavivirus specific nested PCR targeting NS5 region. From August 2018, the colorimetric test was replaced by fluorimetric RDTs: STANDARD F Dengue NS1 Ag FIA + STANDARD F Dengue IgM/IgG FIA (SD Biosensor, Gyeonggi-do, Republic of Korea). RDTs have been performed on samples derived from 651 individuals, representing the 80.9% of possible cases tested at the Institute.Ī colorimetric test detecting the NS1 viral antigen and anti-DENV IgM and IgG (SD Bioline Dengue Duo, Abbott Diagnostics, Abbott Park, IL, USA) was used as preliminary assay between January 2014 and December 2017. DF diagnosis was confirmed at the Regional Reference Laboratory in 102 out of 804 (12.7%) febrile patients tested. Infectious Disease specialists at the Lazzaro Spallanzani National Institute for Infectious Diseases (Rome, Italy) retrospectively reviewed patients with dengue-like symptoms who spontaneously sought treatment in healthcare units of Lazio Region (Central Italy) from January 2014 through July 2019. The use of rapid diagnostic tests (RDTs) may be of help as a preliminary user-friendly quick assay to facilitate dengue diagnosis. Isolation is cumbersome, requires high biocontainment and often takes too long for clinical management. The laboratory diagnosis, essential to recognize dengue cases, is based on viral isolation, serological tests, and detection of viral RNA. Dengue is common in more than 120 countries. The disease can be asymptomatic in about 75% of cases, causing Dengue fever (DF), or result in severe dengue. Hernández-Ortiz, GHI One Health Colombia, Universidad Nacional de Colombia, Medellín, Colombia, and Department of Materials and Nanotechnology, Universidad Nacional de Colombia, Medellin, Colombia, E-mail: Jorge E.Dengue is a mosquito-borne infection caused by four distinct viruses (DENV 1-4) which belong to the flavivirus genus and may lead to diverse outcomes. Berg, Infectious Diseases Research, Abbott Diagnostics, Abbott Park, IL, E-mails: and Juan P. Flórez, and Andres Cardona, GHI One Health Colombia, Universidad Nacional de Colombia, Medellín, Colombia, E-mails: and Gavin A. Ciuoderis, Jaime Usuga, Isabel Moreno, Laura S. Raw data and other additional information are available from the corresponding author upon request.Īuthors’ addresses: Karl A. For participants younger than 18 years, minor assent was also obtained, and parents or a legal representative provided written informed consent on their behalf.ĭata availability: The authors confirm that the data supporting the findings of this study are available within the article and its supplemental materials. Voluntary participation in this study occurred after signing written informed consent. This study was approved by the Research and Ethics Committee of the Corporación Investigaciones Biológicas (No. are employees and shareholders of VaxThera. †These authors contributed equally to this work.įinancial support: This work was supported by the University of Wisconsin-Madison GHI One Health Colombia, Universidad Nacional de Colombia (Medellin), and the Abbott Pandemic Defense Coalition.ĭisclosure: G. We report the detection, isolation, and whole-genome sequencing (11 Kb) of the DENV-2 cosmopolitan genotype and its recent introduction to Colombia. Phylogenetic analyses of the DENV-2 sequences revealed that 42 of 50 of the isolates (84%) belonged to the DENV-2 cosmopolitan genotype lineage, clustering with sequences from Asia, Peru, and Brazil. Three DENV serotypes (1, 2, and 3) were identified. The virus was isolated in cell culture from 166 of these patients (44.1%), and genome sequencing was performed successfully on 122 (73.5%). Combined reverse transcription–polymerase chain reaction and antigen rapid testing confirmed that 376 patients (8.3%) had DF. As part of a hospital-based fever surveillance study, acute-phase sera were collected from 4,545 patients with suspected dengue between 20 in three municipalities of Colombia. In 2022, the Colombian health surveillance system reported more than 69,000 cases of DF. Dengue virus (DENV) is the etiological agent of dengue fever (DF), which is among the most prevalent vector-borne diseases in the tropics.
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